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Beyotime ratiometric fluorescent probe jc 1
Anti‐ferroptotic effects of Na 2 SeO 3 in vitro. (A) Representative images of <t>JC‐1</t> staining (scale bar = 100 μm). (B) Total intracellular iron content. (C) mRNA expression levels of ferroptosis‐related genes. (D) Protein levels of GPX4, COX2, and SLC7A11. (E) Quantitative analysis of ferroptosis‐related proteins shown in (D). Data are expressed as the mean ± SD, n = 3; * p < 0.05, ** p < 0.01 versus control; ns indicates not significant.
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Anti‐ferroptotic effects of Na 2 SeO 3 in vitro. (A) Representative images of <t>JC‐1</t> staining (scale bar = 100 μm). (B) Total intracellular iron content. (C) mRNA expression levels of ferroptosis‐related genes. (D) Protein levels of GPX4, COX2, and SLC7A11. (E) Quantitative analysis of ferroptosis‐related proteins shown in (D). Data are expressed as the mean ± SD, n = 3; * p < 0.05, ** p < 0.01 versus control; ns indicates not significant.
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Beyotime potentiometric fluorescent probe jc 1
Anti‐ferroptotic effects of Na 2 SeO 3 in vitro. (A) Representative images of <t>JC‐1</t> staining (scale bar = 100 μm). (B) Total intracellular iron content. (C) mRNA expression levels of ferroptosis‐related genes. (D) Protein levels of GPX4, COX2, and SLC7A11. (E) Quantitative analysis of ferroptosis‐related proteins shown in (D). Data are expressed as the mean ± SD, n = 3; * p < 0.05, ** p < 0.01 versus control; ns indicates not significant.
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Anti‐ferroptotic effects of Na 2 SeO 3 in vitro. (A) Representative images of JC‐1 staining (scale bar = 100 μm). (B) Total intracellular iron content. (C) mRNA expression levels of ferroptosis‐related genes. (D) Protein levels of GPX4, COX2, and SLC7A11. (E) Quantitative analysis of ferroptosis‐related proteins shown in (D). Data are expressed as the mean ± SD, n = 3; * p < 0.05, ** p < 0.01 versus control; ns indicates not significant.

Journal: Journal of Cellular and Molecular Medicine

Article Title: GPX4 ‐Mediated Inhibition of Ferroptosis by Sodium Selenite Ameliorates Bone Metabolic Disorders in Ovariectomized Mice

doi: 10.1111/jcmm.71414

Figure Lengend Snippet: Anti‐ferroptotic effects of Na 2 SeO 3 in vitro. (A) Representative images of JC‐1 staining (scale bar = 100 μm). (B) Total intracellular iron content. (C) mRNA expression levels of ferroptosis‐related genes. (D) Protein levels of GPX4, COX2, and SLC7A11. (E) Quantitative analysis of ferroptosis‐related proteins shown in (D). Data are expressed as the mean ± SD, n = 3; * p < 0.05, ** p < 0.01 versus control; ns indicates not significant.

Article Snippet: Mitochondrial polarization was assessed using the ratiometric fluorescent probe JC‐1 (Beyotime Biotechnology).

Techniques: In Vitro, Staining, Expressing, Analysis, Control